Genotyping Lupinus angustifolius cultivars with SRAP molecular markers and degenerate primers
نویسندگان
چکیده
منابع مشابه
Precursors of storage proteins in Lupinus angustifolius.
The proteins that are synthesized during differentiation and development in the cotyledons of Lupinus angustifolius L. were characterized both in situ and after purification. The proteins present in situ were separated by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and subjected to 'Western'-blot analysis to identify immunologically related polypeptides. The major storage protein...
متن کاملThe aspartate aminotransferase-P2 gene from Lupinus angustifolius.
and plays a key role in carbon and nitrogen metabolism in plants. The enzyme has been shown to be involved in the shuttling of reducing equivalents from the cytoplasm to chloroplasts, mitochondria, glyoxysomes, and peroxisomes via the malate-aspartate shuttle (Wightman and Forest, 1978). Up to five separate isoforms of AAT have been reported in plants, differing both kinetically and in levels a...
متن کاملAlkaloid Content Variations in Lupinus Luteus L. and Lupinus Angustifolius L
Testing of lupine varieties for alkaloids was performed in the period 2006-2010 at the Voke Branch of the Lithuanian Institute of Agriculture in the course of a competitive trial of feeding lupine (Lupinus sp.). Samples of feeding yellow lupine (Lupinus luteus L.) varieties as well as narrow-leaved lupine (Lupinus angustifolius L.) were used. The alkaloid concentration was assessed in the perio...
متن کاملThe energy value of Lupinus angustifolius and Lupinus albus for growing pigs
Ninety male crossbred pigs were allocated at 30 kg live weight to a 6 5 factorial experiment involving six diets and five levels of feeding where average daily intakes were 1.11, 1.36, 1.67, 1.90 kg and ad libitum between 30 and 60 kg live weight. The control diet contained predominantly animal protein sources, another four diets contained 350 g/kg of either kernels or seeds of either L. angust...
متن کاملSelecting Degenerate Multiplex PCR Primers
Single Nucleotide Polymorphism (SNP) Genotyping is an important molecular genetics technique in the early stages of producing results that will be useful in the medical field. One of the proposed methods for performing SNP Genotyping requires amplifying regions of DNA surrounding a large number of SNP loci. In order to automate a portion of this method and make the use of SNP Genotyping more wi...
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ژورنال
عنوان ژورنال: The EuroBiotech Journal
سال: 2017
ISSN: 2564-615X
DOI: 10.24190/issn2564-615x/2017/02.18